Determination of the specific binding constants of CD45-FITC to protein A by SPR

ZHANG Hui; XUE Hongbao; CHEN Feijian; WANG Jie; WANG Chong; LI Baiqing

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Determination of the specific binding constants of CD45-FITC to protein A by SPR

更新时间：2023-12-11

- Determination of the specific binding constants of CD45-FITC to protein A by SPR
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 57, Issue 4, Pages: 145-149(2018)
- 作者机构：
  
  1. 蚌埠医学院化学教研室,安徽,蚌埠,233030
  2. 
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2018，
  
  Published Online：25 July 2018，
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ZHANG Hui, XUE Hongbao, CHEN Feijian, et al. Determination of the specific binding constants of CD45-FITC to protein A by SPR. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 57(4):145-149(2018)
DOI：

ZHANG Hui, XUE Hongbao, CHEN Feijian, et al. Determination of the specific binding constants of CD45-FITC to protein A by SPR. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 57(4):145-149(2018) DOI：

摘要

运用表面等离子共振（SPR）平台建立测定CD45-FITC抗体与蛋白A特异性结合常数的方法。当偶联蛋白A的传感芯片上流过CD45-FITC抗体溶液时，双通道SPR仪实时监测CD45-FITC抗体与蛋白A特异性结合过程，获得了该反应不同温度下的结合速率常数

、解离速率常数

和结合平衡常数

以及该反应的活化能

和焓变△

。结果表明该特异性结合比较适宜的条件是：中性或微碱性、较高的离子强度和较高的温度。该方法操作步骤简单、快速、灵敏度高、消耗样品少，是研究分析免疫球蛋白 IgG 抗体和蛋白 A 等生物分子相互作用比较理想的方法之一。

Abstract

A SPR method was established to determine the specific binding constants of CD45-FITC to protein A. The specific binding of CD45-FITC was monitored in real time on the SR7500DC Dual Channel System. The characteristic constants for this reaction they were association rate constant

dissociation rate constant

association equilibrium constant

activation energy

and enthalpy change △

. The results indicated that the optimum reaction conditions were under neutral or alkaline medium

with a slightly higher ionic strength and temperature. This method has the characteristics of being fast

simple operation

high sensitivity and less sample

which can be suitable for the investigation of biomolecular interactions of immunoglobulin IgG with protein A.

关键词

表面等离子共振CD45-FITC抗体蛋白A特异性结合常数

Keywords

surface plasmon resonance(SPR)CD45-FITCprotein Aspecificity binding constants

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